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In default of a written contract, the parties try to show in the court the terms and conditions by hearing witnesses People often have very different views, impressions and understandings on what their mutual intention had been earlier Therefore, it is very dif cult to construct afterwards a coherent expression on an agreement that has not been articulated properly before The judge needs to make dif cult deductions which may lead to unsatisfying rulings Having said that, ad-hoc communities hardly ever have carefully drafted written contracts The very basic nature of ad-hoc communities suggest that they do not cautiously prepare documents Therefore, it has to be assumed that ad-hoc communities usually are: contractual arrangements; without written contracts Even if that sounds contradictory, the legal analyses of ad-hoc communities must be based on their vague contractual nature.

(k) Grow cells in a humidi ed incubator at 37 C with 10% CO2 . (l) Passage cells at subcon uence with 0.25% trypsin-EDTA.

If ad-hoc communities are becoming more signi cant in society, the lawmaker is required to set down general rules to reduce uncertainty Sometimes, the contractual nature of a community can get a more speci c form because of the activities that the community performs, if they are governed by special legislation Especially, if the community is involved in gainful employment, it is possible that labour and employment law is to be applied If a community as a whole has committed to work for an employer, it can be possible to say that the community is a work team and that the labour law applies to it In that case, the employer has the right to direct and control the team s work.

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Paper towel Razor blade Microcentrifuge tubes, 2 ml, with screw cap Needle, 20 G Water bath at 60 C Para lm Vortex mixer Photometer and cuvettes Protocol A. Scaffold (a) Grow MSCs on scaffolds in spinner asks as described in Protocol 13.9. (b) Dry the scaffold on a clean paper towel for 3 min on both sides. (c) Measure wet weight of the scaffolds. Eventually cut scaffolds into halves and note the wet weight of halves as well. (d) Put each scaffold into a labeled microcentrifuge tube. (e) Punch 10 little holes into the cap of a microcentrifuge tube. (f) Freeze constructs at 80 C overnight. (g) Lyophilize for 2 3 days and measure the dry weight of each scaffold. (h) Add 1 ml papain solution per tube. (i) Close tube with a new cap; wrap with Para lm. (j) Incubate at 60 C for 16 h, vortexing 5 times. (k) Centrifuge at 3000 g for 10 min at 4 C. (l) Perform GAG assay with supernate. B. Pellets (a) Grow MSCs in pellets according to Protocols 13.7 or 13.8. (b) Centrifuge tube with cell pellet at 3000 g for 10 min at 4 C. (c) Aspirate supernate. (d) Punch 10 little holes into the cap of a microcentrifuge tube. (e) Freeze constructs at 80 C overnight. (f) Lyophilize for 2 3 days. (g) Add 0.5 ml of papain solution per tube. (h) Close tube with a new cap; wrap with Para lm. (i) Incubate at 60 C for 16 h, vortexing 5 times. (j) Centrifuge at 3000 g for 10 min at 4 C. (k) Perform GAG assay with supernate. C. GAG Assay Note: DMMB solution must be checked before use. Keep indicated times constant. (a)

In the relationship between the work team and the employer, the spearhead of the team or the foreman has the authority to negotiate on behalf of the other members It also means that the employer is entitled to bene t.

the three types of molecules considered: non-volatile material (no molecules in the gas phase), which is con ned to the particle; semi-volatile material (this can exist as vapour or condensate); volatile material (e.g. gases which dissolve in the particle). In each layer, molecules are allowed to have production (P ) and loss (L ) rates from chemical reactions with other molecules. Adsorption sites on the surface and their coverage can be treated. We discuss adsorption rates in relation to the sticking probability, Sp , we have used in the next section.

Figure 1.11 (a) Four-hour averages of number (3 800 nm) and mass concentrations (<10 m) between 26 October and 2 November in New Delhi, India. Left axes are for number concentration (dashed line) and right axes for mass concentration (solid line). (b) Four-hour averages of total number (3 800 nm) and mass concentration (<10 m) in Beijing, China, from 26 31 July 2004 (Monday to Saturday). (Reproduced from [60] with permission from Springer Science and Business Media.)

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