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Dreamweaver has greatly improved the New Document interface. You can now quickly choose which type of document you want to create, as well as select from built-in page designs and CSS. The New Document dialog appears each time you press Control+N (Command+N) or choose File New. Use the New Document preferences (shown in Figure 4-9) to refine how you interact with this New Document dialog box.

Protocol 11.7. Selection of Corneal Stromal Stem Cells by Hoechst 33342 Ef ux Reagents and Materials Sterile or aseptically prepared Stromal cells at passages 2 4 HBSS/2FB (see Section 11.2.9) Hoechst 33342 dye, 1 mg/mL in water Propidium iodide, 2 mg/mL in water Verapamil, 500 g/mL in water DMEM/2FB (see Section 11.2.10) Trypsin/TrypLE: TrypLE Express or 0.25% trypsin in CMF-Saline G MoFlo (or similar) high-speed cell sorter, with 350-nm excitation Procedure (a) Trypsinize passage 2 4 stromal cells. (b) Count the cell number and dilute to 1.0 106 cells/mL in DMEM/2FB. (c) Add 5 g/mL Hoechst 33342 dye for 90 min at 37 C; agitate every 20 min. (d) As a control, some cells are preincubated for 20 min with 50 g/mL verapamil before Hoechst 33342 incubation. (e) After staining, wash the cells twice by centrifugation in HBSS/2FB at 4 C and then resuspend and store them in cold HBSS/2FB on ice. (f) Immediately before sorting, add 2 g/mL propidium iodide to identify nonviable cells. (g) Sort cells on a sterile, high-speed cell sorter, using 350-nm excitation. Collect the cells showing reduced uorescence of both blue (670 nm) and red (450 nm). This side population is collected separately from dead cells and from fully labeled cells. As a control, con rm that the side population is eliminated by verapamil preincubation.

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1. Click the Header part indicator to select it. After you ve selected a part indicator, you can apply a color to it. 2. Click and hold down the Fill Color tool in the status area and select a deep shade of navy blue from the Fill Color palette that appears (see Figure 4-17).

Using wild cards to specify multiple files for processing is very powerful. But there are still limitations. You can only process the files in the current directory. Most of the time, I like to sort my photos into their discrete directories prior to processing them. For example, Figure 3-24 shows a list of my photo folders sorted by event. You won t be able to use your GUI application or dcraw to process all of the raw files in all of the folders without manually selecting all the raw files in each directory. But with a command-line interface, dcraw, and a script (a program) you can easily process all of the raw files in all of the folders. The following code is a simple shell script named ProcessRaw.sh that would allow you process all of the raw files in all of the subdirectories. It recursively enters each subdirectory and process all of the raw files in them. The script is recursive because it calls itself multiple times. When all of the directories have been visited, the recursion terminates. This script is very simple (only 12 lines), yet it demonstrates the power of the shell command-line interface. It s possible to write even more sophisticated scripts to accomplish more complex automation tasks. But I ll leave those up to you.

public int g e t v a l u e ( i n t r ) { / / r e a d e r r r e a d s / / r e a d y o u r own r e g i s t e r SRSW t s v = V [ r ] ; // t s v i s l o c a l

South Carolina u. CmseZs,Beaufort County, General Session Indictment No. 2002 GF 070372 (2003) FZorida u. Ubals, 17th Judicial Circuit, Broward County, Case No. 1017144CF 1OA (2003) California v Sanchez, Ventura County, Case No. 2001 9000 34 (2003) Michigan v. Wood, Charlotte, Eaton County, Case No. 02 283 FH (2003) South Dakota u. Junklow, 3rd Cir., Moody County, Case No. 03-147 (2003) New York v. Christmann,Newarkvillage Court, Case No. 03110007 (2004) Arizona u. O Brien, Superior Court of Arizona, Maricopa County, Case No. CR 2003-016197-001DT (2004) Pennsylvania u. Weaver,Bucks County Juvenile Court (2004)

Not everything in spreadsheets revolves around formulas. Comments are useful for annotating a spreadsheet. You may want more control over how a comment appears.

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