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Controversies in the management of germ cell tumours of the ovary Curr Opin Oncol 2006;18:500 506 8 Adamah DJ, Gokhale PJ, Eastwood DJ, et al Dysfunction of the mitotic:meiotic switch as a potential cause of neoplastic conversion of primordial germ cells Int J Androl 2006;29:219 227 9 Surti U, Hoffner L, Chakravarti A, Ferrell RE Genetics and biology of human ovarian teratomas I Cytogenetic analysis and mechanism of origin Am J Hum Genet 1990;47:635 643 10 Houldsworth J, Korkola JE, Bosl GJ, Chaganti RS Biology and genetics of adult male germ cell tumors J Clin Oncol 2006;24:5512 5518 11 Speleman F, De Potter C, Dal Cin P, et al i(12p) in a malignant ovarian tumor Cancer Genet Cytogenet 1990;45:49 53 12 Mutter GL, Lin MC, Fitzgerald JT, et al Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers J Natl Cancer Inst 2000;92:924 930 13.

Procedure GraphPartition EulerPath(G,k) Input: An undirected graph G and grooming factor k. Output: A partition E1 , ..., Ewup of E(G) s.t. |Ei | k. begin Adding dummy edges into G to make each node of G having even degree. Finding an Euler path of G. Partition the Euler path into subgraphs, each of which contains exactly k real edges of G. end.

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Malignant GCTs and sex cord stromal tumors (such as granulosa cell tumors of the ovary) secrete many different serum tumor markers. When frozen section cannot differentiate between tumor types, markers for both suspected diseases should be obtained as soon as possible (even in the operating room if feasible). For germ cell tumors, AFP, hCG, and LDH may helpful in the follow-up of the patient (Table 12.2). In a young patient, CA-125 will not be useful, as the likelihood of an epithelial ovarian cancer is very small, and this value can be falsely elevated (Table 12.5). Serum total inhibin is a very speci c tumor marker for granulosa cell tumors and should be used to follow patients with this disease.2

Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill, NC, USA

Di Vizio D, Cito L, Boccia A, et al Loss of the tumor suppressor gene PTEN marks the transition from intratubular germ cell neoplasias (ITGCN) to invasive germ cell tumors Oncogene 2005;24:1882 1894..

Although the effects of xenobiotics described in the previous chapters are very serious and range from molecular damage and cancer development to disease and death of individuals and reduction in population abundance, they are usually short-lived and quickly disappear once exposure has stopped. However, effects on the population genetic level may impact populations for many generations to come. For example, once genetic diversity is lost from a population, it will in the absence of immigration only be replenished on evolutionary time scales. Similarly, certain deleterious mutations accumulating in the germline may take many generations to be eliminated by selection. Considerable concern has developed over such potential chronic and transgenerational effects of contamination (Bickham et al., 2000), and their consequences for population productivity and persistence. While much of the theory of mutation accumulation, selection and loss of genetic diversity is well developed, there is still a great need to: (1) (2) (3) Establish rm links between damage at the molecular level, and long-term genetic changes at the population level Investigate the speed, extent and consequences of genetic adaptation to pollution Study the consequences of xenobiotics on the genetic diversity of exposed populations.

Rapid developments in VLSI over the last decades have resulted in IC chips consisting of millions of devices and interconnections. In addition, the reductions of device and other dimensions have led to ever-increasing complexity of these chips. With the scaling of the device and interconnection dimensions, several factors need to be considered that have important bearings on the reliability of the chip. These include the small geometry effects in MOSFETs and MESFETs such as hot-carrier effects, dielectric breakdown, electromigration effects in the interconnections, and radiation effects. The reliability performance of a VLSI chip is of major concern to a designer. In the past, because of the lack of CAD tools, VLSI reliability tests were limited to the

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