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Most useful restriction enzymes cut DNA at specific recognition sites, usually four to six nucleotides in length There can be multiple restriction sites for a single endonuclease within a given piece of DNA, there can be only one (a unique restriction site), or there can be none It all depends on the sequence of the specific piece of DNA in question Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place It s also a useful way to name pieces of DNA For example, a piece of DNA that is cut from a bigger piece of DNA is often named by size and given a surname that corresponds to the two restriction enzymes that did the cutting the 03-kb EcoRI-BamHI fragment Restriction enzymes themselves are named for the bacterial strains from which they were initially isolated A restriction map shows the location of restriction sites in a given DNA sequence When digested with two (or more) restriction enzymes at the same time, most large pieces of DNA give a specific pattern of different-sized DNA fragments depending on the distance separating the different cleavage sites These different fragments can then be separated by size on an agarose gel By working backward (biochemists are good at this) from the sizes of the different DNA fragments, it is possible to construct a map that locates the different restriction sites along a given piece of DNA For example, if we cut the 36-kb piece of DNA in Fig 6-1 with SmaI, we would see two bands on the agarose gel 19 and 17 kb This would tell us that the SmaI site is very near the middle of the fragment We could start constructing our map by putting the 17-kb fragment on the left side or the right side it doesn t matter, and we can t know which is right (or left) In Fig 6-1, the DNA is arbitrarily put down with the smaller fragment on the right If we cut with BamHI, we get fragments that are 09 and 27 kb Again we wouldn t know whether to put the BamHI site on the right or left of the map, but here it does matter because we already have the SmaI site on the map The way to decide where to put the BamHI site is to cut with both BamHI and SmaI Let s say that you get fragments of 09, 10, and 17 kb Notice that the 17-kb fragment is the same size as in the digest with SmaI alone This tells you that the BamHI site is in the 19-kb SmaI fragment, that is, on the left side of our map By going through this kind of reasoning over and over, it is possible to construct a map of restriction sites along your piece of DNA Restriction enzymes that recognize a specific sequence of five nucleotides should cut the DNA, on average, every 45 base pairs (this is the frequency with which a given sequence of five nucleotides would occur by chance), or every 1024 base pairs As a result, the average size.

Encyclopedia Britannica Online, wwwbritannicacom Eric s Treasure Troves of Science, wwwtreasure-trovescom Sky and Telescope, wwwskypubcom Weather Underground, wwwwundergroundcom

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It s easy to understand how using signs reduces frustration One of the other well-established benefits, however, may surprise you Babies taught signs actually learn to speak sooner and have richer vocabularies In one of our studies, the signing babies on average knew about fifty more real words than their nonsigning peers by the age of two Moreover, these gains did not disappear as time went on A year later, at age three, the signers were both saying and understanding words at levels almost comparable to what is expected at age four! Why does the Baby Signs program help babies master language more quickly Here are a few possible explanations: Food for thought for the brain Babies come into this world with a mind-boggling 100 to 200 billion brain cells (or neurons) What they don t come into the world with are the trillions and trillions of connections among these neurons These connections, as much as the neurons themselves, are what enable them to organize thoughts, see relationships among things, remember past events, and master language How do these connections come about Both their creation and their continued existence depend a great deal on a child s experiences in the world The more often a child encounters thought-

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provoking objects, events, and problems, the more connections are made and strengthened This general principle is clearly relevant to the relation between signing and learning to talk Every time a baby successfully uses a sign, changes occur in the brain, bringing the child closer to mastering language The circuitry in the brain which talking requires develops along with a child s experience with language Because using signs enables children to begin the process earlier, the development of this circuitry gets a significant jump start that continues to pay off for years down the road Like crawling is to walking Just as babies learn to crawl before they can walk, using signs gives them a developmentally appropriate way to communicate before they can talk Once children learn to walk, they no longer crawl because of the greater freedom walking affords them Communication is the same way While signs are useful before children have words, speech allows them the ability to communicate more quickly and more fully As your child s mind and body develop, she will naturally transition to speaking in order to convey ever more complex ideas and longer sentences Far from getting in the way of the process, using the Baby Signs program provides a bridge that helps the transition from no language to spoken language A tutorial for talking The experience of signing teaches babies useful lessons about how language works lessons that speed up the process of learning to talk once words are finally available By enabling a baby to practice learning and using symbols to label objects, express needs, and describe feelings, signing creates the mental framework that makes it easy to incorporate words as soon as the baby s vocal chords are developed enough to use them

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