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Fig. 4.6 Labeled plotting elds on an Expanded Durov diagram (cf. Figure 4.4b), which can be used to identify different hydrochemical facies in groundwater systems. The nine numbered elds in the central plotting square are identi ed and explained in Table 4.3. If plotted samples along a groundwater ow line (as de ned on a ow net) show a clear trend from one facies to another, then it is possible to infer the predominant processes affecting the evolution of groundwater chemistry. Two examples of trend lines are shown here: Trend A is typical of ion-exchange during groundwater ow (as explained in Section 4.4.2), while Trend B is typical of mixing between fresh and saline groundwaters (see Section 4.4.3).

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In human terms we think of motivation as a desire to do something. We would probably make a distinction between calculated rational motivations with a conscious goal and more impulsive motivations driven by an internal urge such as hunger or fear. In fact we should probably consider these to be the extremes of a continuum of motivational types.

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Masters, JR (2002) HeLa cells 50 years on: the good, the bad and the ugly Nat Rev Cancer 2: 315 9 Masters, JRW, Thomson, JA, Daly-Burns, B, Reid, YA, Dirks, WG, Packer, P, Toji, LH, Ohno, T, Tanabe, H, Arlett, CF, Kelland, LR, Harrison, M, Virmani, A, Ward, TH, Ayres, KL, Debenham, PG (2001) STR pro ling provides an international reference standard for human cell lines Proc Natl Acad Sci USA 98: 8012 8017 Mather, JP (1998) Making informed choices: medium, serum, and serum-free medium How to choose the appropriate medium and culture system for the model you wish to create Methods Cell Biol 57: 19 30 Michel, M, L Heureux, N, Pouliot, R, Xu, W, Auger, F.

A, Lucie, G (1999) Characterization of a new tissue-engineered human skin equivalent with hair In Vitro Cell Dev Biol Anim 35: 318 326 Munger, K, Howley, PM (2002) Human papillomavirus immortalization and transformation functions Virus Res 89(2): 213 228 Peehl, DM, Ham, RG (1980) Clonal growth of human keratinocytes with small amounts of dialysed serum In Vitro 16: 526 540 Petra, M, de Jong, PM, van Sterkenburg, AJA, Kempenaar, JA, Dijkman, JH, Ponec, M (1993) Serial culturing of human bronchial epithelial cells derived from biopsies In Vitro Cell Dev Biol 29A: 379 387 Pretlow, TG, and Pretlow, TP (1989) Cell separation by gradient centrifugation methods Methods Enzymol 171: 462 482 Raff, MC (1990) Glial cell diversi cation in the rat optic nerve Science 243: 1450 1455 Regnier, M.

, Staquet, MJ, Schmitt, D, Schmidt, R (1997) Integration of Langerhans cells into a pigmented reconstructed human epidermis J Invest Dermatol 109: 510 512 Rizzino, A (2002) Embryonic stem cells provide a powerful and versatile model system Vitam Horm 64: 1 42 Saalbach, A, Aust, G, Haustein, UF, Herrmann, K, Anderegg, U (1997) The broblastspeci c MAb AS02: A novel tool for detection and elimination of human broblasts Cell Tissue Res 290: 593 599 Schaller, M, Mailhammer, R, Grassl, G, Sander, CA, Hube, B, Korting, HC (2002) Infection of human oral epithelia with Candida species induces cytokine expression correlated to the degree of virulence J Invest Dermatol 118: 652 657 Stoker, MGP (1973) Role of diffusion boundary layer in contact inhibition of growth Nature 246: 200 203 Swope, VB, Supp, A.

Note: The results of this subsection follow from the rules of the previous sections of this chapter and the rules for determinants (see also Magnus (1988, 8 .

Uncompressed images can be large. Consider Table 37-1, which compares image dimensions, number of colors, and file size for some sample uncompressed images. As you can see, with file sizes like this, you would have to limit yourself to mighty tiny images, or two-color, such as black and white, images. Or, you could compress the files.

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